Färbungen


Konventionell-histologische Färbung	Immunhistochemie (IHC)	In Situ Hybridisierung (ISH)

Abkürzung m: monoklonaler Antikörper p: polyklonaler Antikörper	Antigen gegen welches der Antikörper gerichtet ist	Angefärbte Struktur, Nachweis

A-b-amyloid	An immunohistochemical staining detecting pathological Ab amyloid.	Accumulates as extracellular Ab plaques (either as "soft" or "core" plaques), mainly in cortical areas of patients with Alzheimer's disease. In addition, Ab may accumulate within cerebral blood vessels resulting in a disease called cerebral amyloid angiopathy (CAA).

a-synuclein	An immunohistochemical staining to a-synuclein.	Aggregated a-synuclein proteins form brain lesions that are hallmarks of some neurodegenerative diseases (so-called synucleinopathies, such as Parkinson's disease (PD) or dementia with Lewy bodies (DLB))

CD20 m	Cluster of Differentiation 20. Membran-Phosphoprotein 35/37 kDa (mAb L26 erkennt eine intrazytoplasmatische Domäne. Funktion: Regulation der Zellaktivierung (via Kalziumionenkanal)	Frühes B-Zell-Antigen, exprimiert bis zum Stadium der reifen Plasmazelle

CD68 m	Cluster of Differentiation 68. Intrazelluläres Glykoprotein, dass mit Monocyten und Gewebsmakrophagen assoziert ist. Identifizierung von Makrophagen.	Antigen auf lysosomalen Membranproteine von Makrophagen, aber auch in Monozyten, Neutrophilen, Basophilen und großen Lymphozyten nachweisbar

CIV 22 m	Typ IV Kollagen (Basalmembrankollagen)	Basalmembranen

Crg A m	Chromogranin A Saures monomeres Protein der Matrix der Sekretgranula endokriner Zellen	Zytoplasma endokriner Zellen

Desmin m	Desmin Polypeptid, MG 51.000	Intermediärfilamente der Muskelzellen

GFAP p	Glial fibrillary acidic protein Polypeptid, MG 50.000	Intermediärfilamente der Astrozyten

HCG-beta	Human chorionic gonadotropin, hormonspezifische Untereinheit, Glykoprotein, 145 Aminosäuren Intakt. Hormon: MG ca. 37.000	Zytotrophoblast, choriale Riesenzellen im Chorionepitheliom

HPV m	Human Papilloma Virus Hüllprotein	Befallene Zellkerne, evtl. Virusantigen im Zytoplasma

Ig m	Immunglobulin Schwere Kette, Typ A, D, G oder M	B-Lymphozyten, Plasmazellen Nachweis der Monoklonalität möglich

Leichtketten m	Leichtketten der Immunglobuline kappa oder lambda	B-Lymphozyten, Plasmazellen Nachweis der Monoklonalität möglich

LU-5 m	Konformationabhängiges Epitop in allen derzeit bekannten 20 Zytokeratinen (Polypeptide), MG 40.000 - 65.000	Zellen mit Zytokeratin-enthaltenden Intermediärfilamenten: "Epithel- und Mesothelzellen"

MAP2C	An immunohistochemical staining using the antibody clone MAP2C detecting the microtubule-associated protein-2 (MAP-2).	MAP-2 is concentrated in neuronal somata and dendrites. also detects: (i) oligodendroglial tumour cells with a perinuclear staining pattern (ii) astrocytic tumour cells with a fibrillary staining pattern

Panzytokeratin	Zytoskeletale Filamente	Alle epithelialen Strukturen

PRL m	Prolaktin; Einkettiges Proteohormon, 198 Aminosäuren, MG ca. 26.400	Prolaktinzellen der Hypophyse bzw. von Hypophysentumoren

PrP	An immunohistochemical staining to detect human prion protein (PrP).	The antibody recognizes both the cellular (PrP^C) and the pathological (PrP^Sc) form of PrP

PSA m	Prostate-specific Antigen Glykoprotein, MG ca. 33.000	Epithelzellen der Prostata bzw. des Prostatakarzinoms

S-100 p	Zytoplasmatische Protein S-100 MG ca. 21.000 S-100 a: a- und b-Untereinheiten S-100 b: 2 identische b-Untereinheiten Antiserum erkennt Epitope auf beiden Untereinheiten	Zytoplasma glialer Zellen, von Schwannzellen, von Satellitenzellen des Nebennierenmarkes, von myoepithelialen Zellen, von Melanozyten, Melanomzellen, Chondrozyten, dendritischen Retikulumzellen u.a.

SP m	Substance P, Peptid (Tachykinin) 11 Aminosäuren, MG 1348	Substance P-erge Neurone, bestimmte enterochromaffine Zellen, Zellen der Ileumkarzinoide

Syn p,m	Synaptophysin, glykosyliertes Polypeptid aus Membranen präsynaptischer Vesikeln, MG 38.000	Neuroendokrine Zellen, neuroendokrine Tumorzellen

Tg m	Thyreoglobulin Glykoprotein, MG ca. 660.000	Follikelepithelzellen der Schilddrüse, follikuläres und papilläres Schilddrüsenkarzinom

Treponema pallidum m	Treponema pallidum Antibody	Reacts with Treponema pallidum in infected tissue samples.

Vim m	Vimentin Polypeptid, MG 55000	Vimentin-enthaltende Intermediärfilamente mesenchymaler Zellen

Abbreviation m: monoclonal antibody p: polyclonal antibody	Antigen, binding to the antibody	Stained structure/detection

A-b-amyloid	An immunohistochemical staining detecting pathological Ab amyloid.	Accumulates as extracellular Ab plaques (either as "soft" or "core" plaques), mainly in cortical areas of patients with Alzheimer's disease. In addition, Ab may accumulate within cerebral blood vessels resulting in a disease called cerebral amyloid angiopathy (CAA).

a-synuclein	An immunohistochemical staining to a-synuclein.	Aggregated a-synuclein proteins form brain lesions that are hallmarks of some neurodegenerative diseases (so-called synucleinopathies, such as Parkinson's disease (PD) or dementia with Lewy bodies (DLB))

CD20 m	Cluster of Differentiation 20. Membrane-phosphoprotein 35/37 kDa (mAb L26 recognizes an intracytoplasmatic domain. Function: Regulation of cell activation (via calcium-ion-channel)	Early B-cell-antigen, expressed up to the state of the mature plasma cell

CD68	Cluster of Differentiation 68. Intracellular glycoprotein, associated with monocytes and tissue macrophages. Identification of macrophages	Antigen to lysosomal membrane proteins of macrophages. Can also be found in monocytes, neutrophils, basophils and large lymphocytes

CIV 22 m	Type IV Collagen (basal lamina collagen)	Basal lamina

Crg A m	Chromogranin A Acidic sulfated glycoprotein found in secretory granules of most endocrine and neuroendocrine cells.	cytoplasm of endocrine cells

Desmin m	Desmin Polypeptide, MW 51.000	intermediate filament of muscle cells

GFAP p	Glial fibrillary acidic protein Polypeptide, MW 50.000	intermediate filament protein of astrocytes

HCG-beta	Human chorionic gonadotropin, hormonspecific subunit, glycoprotein, 145 amino acids Intact hormone: MW ca. 37.000	cytotrophoblast, chorial giant cells in chorionepithelioma

HPV m	Human Papilloma Virus envelope protein	affected nuclei, possibly virus-antigen in cytoplasm

Ig m	Immunglobulin heavy chain, type A, D, G or M	B-Lymphozyten, B-lymphocytes, plasma cells proof of monoclonality possible

light chains m	light chains of immunoglobulins kappa or lambda	B-lymphocytes, plasma cells proof of monoclonality possible

LU-5 m	conformation-dependent epitope in all presently known 20 cytokeratins (polypeptides), MW 40.000 - 65.000	cells with cytokeratin-containing intermediate filaments: "epithelial and mesothelial cells"

MAP2C	An immunohistochemical staining using the antibody clone MAP2C detecting the microtubule-associated protein-2 (MAP-2).	MAP-2 is concentrated in neuronal somata and dendrites. also detects: (i) oligodendroglial tumour cells with a perinuclear staining pattern (ii) astrocytic tumour cells with a fibrillary staining pattern

Pancytokeratin	cytoskeletal filaments	all epithelial structures

PRL m	Prolactin; one chained proteohormone, 198 amino acids, MW ca. 26.400	Prolactin-cells of hypophysis respectively of pituitary tumors

PrP	An immunohistochemical staining to detect human prion protein (PrP).	The antibody recognizes both the cellular (PrP^C) and the pathological (PrP^Sc) form of PrP

PSA m	Prostate-specific Antigen Glycoprotein, MW ca. 33.000	epithelial cells of prostate respectiveley of carcinoma of the prostate

S-100 p	cytoplasmatic protein S-100 MW ca. 21.000 S-100 a: a- and b-subunits S-100 b: 2 identical b-subunits Antiserum recognizes epitopes on both subunits	cytoplasm of glial cells, Schwann's cells, satellite cells of the adrenal medulla, myoepithelial cells, melanocytes, melanoma cells, chondrocytes, dendritic reticulum cells et al.

SP m	Substance P, peptide (Tachykinin) 11 amino acids, MW 1348	Substance P-ergic neurons, certain enterochromaffin cells, cells of ileum carcinoids

Syn p,m	Synaptophysin, glycosylated polypeptide of membranes of presynaptic vesicles, MW 38.000	neuroendocrine cells, neuroendocrine tumor cells

Tg m	Thyroglobulin Glycoprotein, MW ca. 660.000	follicular epithelial cells of the thyroid, follicular and papillary thyroid carcinoma

Vim m	Vimentin Polypeptide, MW 55000	Vimentin-containing intermediate filaments of mesenchymal cells